In Vitro Anthelmintic Effect of Ferula assa-foetida Hydroalcoholic Extract Against Flukes of Fasciola hepatica and Dicrocoelium dendriticum

Background: Dicrocoeliasis and fascioliasis are foodborne parasitic diseases of the biliary tract, resulting from Dicrocoelium dendriticum Fasciola hepatica causing extensive financial losses serious health problems in ruminants. Due to low-performance medications, drug delivery is a tremendous project improve interventions available for these diseases. Objectives: This study aimed determine anthelmintic properties Ferula assa-foetida extract against F. D. using vitro assay. Methods: The effects diverse concentrations (400 - 1000 µg/mL) 12-24 hours were examined treatment hepatica. efficacies evaluated scanning electron microscopy (SEM). MTT assay was carried out evaluate cell viability all cells culture media. Results: SEM images treated worms by (200 confirmed excessive damage, which included an entire lack sensory papillae destruction distinguished network structures tegument vesicles. Variables duration concentration presented considerable effect on both mortality rate assa-foetida; accordingly, as time increased, became higher. Based assay, toxicity at 800 µg/mL 8.7%. Therefore, it can be argued that had properties. Conclusions: first impact liver flukes paved way further studies control those trematodes. It recommended document look into indigenous understanding viable medicinal plants provide evidence their use.